Braindead
04-16-2007, 01:36 AM
I take my green tea extract and zocor and hope the prions will stay away.
.......
1: J Virol. 2003 Oct;77(19):10288-94.
New inhibitors of scrapie-associated prion protein formation in a library of
2000 drugs and natural products.
Kocisko DA, Baron GS, Rubenstein R, Chen J, Kuizon S, Caughey B.
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories,
National Institute of Allergy and Infectious Diseases, National Institutes
of Health, Hamilton, Montana 59840, USA.
Transmissible spongiform encephalopathies (TSEs) are fatal, untreatable
neurodegenerative diseases associated with the accumulation of a
disease-specific form of prion protein (PrP) in the brain. One approach to
TSE therapeutics is the inhibition of PrP accumulation. Indeed, many
inhibitors of the accumulation of PrP associated with scrapie (PrP(Sc)) in
scrapie-infected mouse neuroblastoma cells (ScN(2)a) also have antiscrapie
activity in rodents. To expedite the search for potential TSE therapeutic
agents, we have developed a high-throughput screening assay for PrP(Sc)
inhibitors using ScN(2)a cells in a 96-well format. A library of 2000 drugs
and natural products was screened in ScN(2)a cells infected with scrapie
strain RML (Chandler) or 22L. Forty compounds were found to have
concentrations causing 50% inhibition (IC(50)s) of PrP(Sc) accumulation of
<or=10 microM against both strains. Seventeen had IC(50)s of <or=1 microM
against both strains.
Several classes of compounds were represented in the 17 most potent
inhibitors, including naturally occurring polyphenols (e.g., tannic acid and
tea extracts), phenothiazines, antihistamines, statins, and antimalarial
compounds.
These 17 compounds were also evaluated in a solid-phase cell-free hamster
PrP conversion assay. Only the polyphenols inhibited the cell-free reaction,
and their IC(50)s were near 100 nM. Several of the new PrP(Sc) inhibitors
cross the blood-brain barrier and thus have potential to be effective after
TSE infection reaches the brain. The fact that many are either approved
human drugs or edible natural products should facilitate their use in animal
testing and clinical trials.
PMID: 12970413 [PubMed - indexed for MEDLINE]
.......
1: J Virol. 2003 Oct;77(19):10288-94.
New inhibitors of scrapie-associated prion protein formation in a library of
2000 drugs and natural products.
Kocisko DA, Baron GS, Rubenstein R, Chen J, Kuizon S, Caughey B.
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories,
National Institute of Allergy and Infectious Diseases, National Institutes
of Health, Hamilton, Montana 59840, USA.
Transmissible spongiform encephalopathies (TSEs) are fatal, untreatable
neurodegenerative diseases associated with the accumulation of a
disease-specific form of prion protein (PrP) in the brain. One approach to
TSE therapeutics is the inhibition of PrP accumulation. Indeed, many
inhibitors of the accumulation of PrP associated with scrapie (PrP(Sc)) in
scrapie-infected mouse neuroblastoma cells (ScN(2)a) also have antiscrapie
activity in rodents. To expedite the search for potential TSE therapeutic
agents, we have developed a high-throughput screening assay for PrP(Sc)
inhibitors using ScN(2)a cells in a 96-well format. A library of 2000 drugs
and natural products was screened in ScN(2)a cells infected with scrapie
strain RML (Chandler) or 22L. Forty compounds were found to have
concentrations causing 50% inhibition (IC(50)s) of PrP(Sc) accumulation of
<or=10 microM against both strains. Seventeen had IC(50)s of <or=1 microM
against both strains.
Several classes of compounds were represented in the 17 most potent
inhibitors, including naturally occurring polyphenols (e.g., tannic acid and
tea extracts), phenothiazines, antihistamines, statins, and antimalarial
compounds.
These 17 compounds were also evaluated in a solid-phase cell-free hamster
PrP conversion assay. Only the polyphenols inhibited the cell-free reaction,
and their IC(50)s were near 100 nM. Several of the new PrP(Sc) inhibitors
cross the blood-brain barrier and thus have potential to be effective after
TSE infection reaches the brain. The fact that many are either approved
human drugs or edible natural products should facilitate their use in animal
testing and clinical trials.
PMID: 12970413 [PubMed - indexed for MEDLINE]