flatfish
10-11-2006, 01:41 PM
##################### Bovine Spongiform Encephalopathy #####################
Prion protein in cardiac muscle of elk (Cervus elaphus nelsoni) and white-tailed deer (Odocoileus virginianus) infected with chronic wasting disease
Jean E. Jewell1, Jeremy Brown1, Terry Kreeger2 and Elizabeth S. Williams1,
1 Department of Veterinary Sciences, University of Wyoming, Wyoming State Veterinary Laboratory (WSVL), 1174 Snowy Range Road, Laramie, WY 82070, USA
2 Veterinary Services Branch, Wyoming Game and Fish Department (WGFD), Wheatland, WY 82201, USA
Correspondence
Jean E. Jewell
jjewell@uwyo.edu
To investigate the possible presence of disease-associated prion protein (PrPd) in striated muscle of chronic wasting disease (CWD)-affected cervids, samples of diaphragm, tongue, heart and three appendicular skeletal muscles from mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginianus), elk (Cervus elaphus nelsoni) and moose (Alces alces shirasi) were examined by ELISA, Western immunoblot and immunohistochemistry (IHC). PrPd was detected in samples of heart muscle from seven of 16 CWD-infected white-tailed deer, including one free-ranging deer, and in 12 of 17 CWD-infected elk, but not in any of 13 mule deer samples, nor in the single CWD-infected moose. For white-tailed deer, PrPd was detected by Western blot at multiple sites throughout the heart; IHC results on ventricular sections of both elk and white-tailed deer showed positive staining in cardiac myocytes, but not in conduction tissues or nerve ganglia. Levels of PrPd in cardiac tissues were estimated from Western blot band intensity to be lower than levels found in brain tissue. PrPd was not detected in diaphragm, triceps brachii, semitendinosus, latissiumus dorsi or tongue muscles for any of the study subjects. This is the first report of PrPd in cardiac tissue from transmissible spongiform encephalopathy-infected ruminants in the human food chain and the first demonstration by immunological assays of PrPd in any striated muscle of CWD-infected cervids.
Deceased 29 December 2004.
http://vir.sgmjournals.org/cgi/content/abstract/87/11/3443?ct
TSS
#################### https://lists.aegee.org/bse-l.html ####################
##################### Bovine Spongiform Encephalopathy #####################
Patterns of PrPCWD accumulation during the course of chronic wasting disease infection in orally inoculated mule deer (Odocoileus hemionus)
Karen A. Fox1,2, Jean E. Jewell3, Elizabeth S. Williams3, and Michael W. Miller1
1 Colorado Division of Wildlife, Wildlife Research Center, 317 West Prospect Road, Fort Collins, CO 80526-2097, USA
2 College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
3 Department of Veterinary Sciences, University of Wyoming, 1174 Snowy Range Road, Laramie, WY 82070, USA
Correspondence
Michael W. Miller
mike.miller@state.co.us
Patterns of abnormal prion protein (PrP) accumulation during the course of chronic wasting disease (CWD) infection were studied and the distribution and timing of disease-associated PrP (PrPCWD) deposition and lesions in 19 mule deer (Odocoileus hemionus) 90–785 days after oral inoculation were described. PrPCWD deposition occurred relatively rapidly and widely in lymphoid tissues, later in central and peripheral nervous tissues and sporadically in a variety of tissues and organs in terminal disease stages. Development of spongiform encephalopathy lagged behind PrPCWD deposition in the central nervous system (CNS), but occurred in the same neuroanatomical locations. PrPCWD deposition in the lymphatic and nervous systems tended to be consistent and progressive in specific organs and tissues. Locations of PrPCWD deposition were similar between deer of two PrP genotypes (225SS and 225SF), but the time course differed between genotypes: in 225SF deer, PrPCWD accumulated more slowly in lymphatic tissues than in 225SS animals, but that disparity was small in comparison to the disparity between genotypes in timing of deposition in CNS tissue. These data confirm retropharyngeal lymph node and medulla oblongata at the level of the obex as early sites of PrPCWD accumulation in mule deer with CWD. Data on the relative time frames for and genetic influences on PrPCWD accumulation may also offer insights about epidemic dynamics and potential control strategies.
Deceased 29 December 2004.
http://vir.sgmjournals.org/cgi/content/abstract/87/11/3451
TSS
#################### https://lists.aegee.org/bse-l.html ####################
Prion protein in cardiac muscle of elk (Cervus elaphus nelsoni) and white-tailed deer (Odocoileus virginianus) infected with chronic wasting disease
Jean E. Jewell1, Jeremy Brown1, Terry Kreeger2 and Elizabeth S. Williams1,
1 Department of Veterinary Sciences, University of Wyoming, Wyoming State Veterinary Laboratory (WSVL), 1174 Snowy Range Road, Laramie, WY 82070, USA
2 Veterinary Services Branch, Wyoming Game and Fish Department (WGFD), Wheatland, WY 82201, USA
Correspondence
Jean E. Jewell
jjewell@uwyo.edu
To investigate the possible presence of disease-associated prion protein (PrPd) in striated muscle of chronic wasting disease (CWD)-affected cervids, samples of diaphragm, tongue, heart and three appendicular skeletal muscles from mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginianus), elk (Cervus elaphus nelsoni) and moose (Alces alces shirasi) were examined by ELISA, Western immunoblot and immunohistochemistry (IHC). PrPd was detected in samples of heart muscle from seven of 16 CWD-infected white-tailed deer, including one free-ranging deer, and in 12 of 17 CWD-infected elk, but not in any of 13 mule deer samples, nor in the single CWD-infected moose. For white-tailed deer, PrPd was detected by Western blot at multiple sites throughout the heart; IHC results on ventricular sections of both elk and white-tailed deer showed positive staining in cardiac myocytes, but not in conduction tissues or nerve ganglia. Levels of PrPd in cardiac tissues were estimated from Western blot band intensity to be lower than levels found in brain tissue. PrPd was not detected in diaphragm, triceps brachii, semitendinosus, latissiumus dorsi or tongue muscles for any of the study subjects. This is the first report of PrPd in cardiac tissue from transmissible spongiform encephalopathy-infected ruminants in the human food chain and the first demonstration by immunological assays of PrPd in any striated muscle of CWD-infected cervids.
Deceased 29 December 2004.
http://vir.sgmjournals.org/cgi/content/abstract/87/11/3443?ct
TSS
#################### https://lists.aegee.org/bse-l.html ####################
##################### Bovine Spongiform Encephalopathy #####################
Patterns of PrPCWD accumulation during the course of chronic wasting disease infection in orally inoculated mule deer (Odocoileus hemionus)
Karen A. Fox1,2, Jean E. Jewell3, Elizabeth S. Williams3, and Michael W. Miller1
1 Colorado Division of Wildlife, Wildlife Research Center, 317 West Prospect Road, Fort Collins, CO 80526-2097, USA
2 College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
3 Department of Veterinary Sciences, University of Wyoming, 1174 Snowy Range Road, Laramie, WY 82070, USA
Correspondence
Michael W. Miller
mike.miller@state.co.us
Patterns of abnormal prion protein (PrP) accumulation during the course of chronic wasting disease (CWD) infection were studied and the distribution and timing of disease-associated PrP (PrPCWD) deposition and lesions in 19 mule deer (Odocoileus hemionus) 90–785 days after oral inoculation were described. PrPCWD deposition occurred relatively rapidly and widely in lymphoid tissues, later in central and peripheral nervous tissues and sporadically in a variety of tissues and organs in terminal disease stages. Development of spongiform encephalopathy lagged behind PrPCWD deposition in the central nervous system (CNS), but occurred in the same neuroanatomical locations. PrPCWD deposition in the lymphatic and nervous systems tended to be consistent and progressive in specific organs and tissues. Locations of PrPCWD deposition were similar between deer of two PrP genotypes (225SS and 225SF), but the time course differed between genotypes: in 225SF deer, PrPCWD accumulated more slowly in lymphatic tissues than in 225SS animals, but that disparity was small in comparison to the disparity between genotypes in timing of deposition in CNS tissue. These data confirm retropharyngeal lymph node and medulla oblongata at the level of the obex as early sites of PrPCWD accumulation in mule deer with CWD. Data on the relative time frames for and genetic influences on PrPCWD accumulation may also offer insights about epidemic dynamics and potential control strategies.
Deceased 29 December 2004.
http://vir.sgmjournals.org/cgi/content/abstract/87/11/3451
TSS
#################### https://lists.aegee.org/bse-l.html ####################